Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Feeling salty: A welfare framework for estuarine fishes
Grantee: Samantha Levell
Institution: New College of Florida, United States
Project summary
Estuaries are highly dynamic environments in which salinity can change rapidly. This project will study how short-term, ecologically realistic salinity shifts influence welfare in estuarine fishes using non-invasive measures such as waterborne cortisol, metabolic rate, exploration, shoaling behavior, and decision making. The project will focus on two common estuarine species: the sheepshead minnow (Cyprinodon variegatus) and the sailfin molly (Poecilia latipinna). By comparing juveniles and adults, the project aims to develop practical tools for assessing fish welfare in the wild and to better understand how environmental variability shapes the experiences of aquatic animals.
Assessing behavioral and physiological welfare indicators for the Kashmir loach (Triplophysa kashmirensis)
Grantee: Misba Rehman
Institution: Sher-e-Kashmir University of Agricultural Sciences and Technology, India
Project summary
This project aims to develop and validate non-invasive behavioral and physiological welfare indicators for the Kashmir loach (Triplophysa kashmirensis). The researchers will use underwater cameras to record behaviors like activity, refuge use, foraging, and abnormal swimming patterns. Physiological welfare will be assessed using mucus sampling to measure cortisol concentrations and supplementary indicators including oxidative stress and immune markers. The team will validate indicators by testing their correlation with key water quality parameters. The project aims to establish the first field-based welfare assessment framework for this species and develop a transferable model for wild freshwater fishes inhabiting mountain stream ecosystems.
Harnessing machine learning for the non-invasive assessment of wild fish welfare
Grantee: Thomas Pike
Institution: University of Lincoln
Project summary
This project aims to develop an open-source aquatic camera system capable of automatically and non-invasively quantifying a suite of behavioural and cognitive welfare indicators in wild fish. Behavioural indicators include the degree of physical closeness between individuals within a group, the spatial arrangement of subgroups, movement behaviour, and agonistic interactions. The project will examine variation in these indicators along an urban–rural gradient to a range of putative stressors, before validating them using “challenge” tests involving ecologically relevant experimental interventions (e.g., acoustic disturbances such as boat noise). The overarching objective is to develop an open-source toolkit that integrates commercially available hardware with custom-designed software, allowing the methods to be readily used by others to assess wild fish welfare.
Stranding: a blessing or a curse? Testing assumptions of fish welfare during habitat fragmentation
Grantee: Laura R. Stein
Institution: University of Oklahoma
Project summary
Fragmentation of freshwater habitats due to drought and heatwaves poses significant risks to aquatic organisms, particularly small fish, who are often overlooked in animal welfare studies. While fragmentation is generally considered detrimental to fish welfare — leading to stress, reduced social interactions, and increased mortality — there is also potential for positive welfare effects, such as reduced predation pressure or temperature-induced metabolic benefits. This project will use threespine stickleback (Gasterosteus aculeatus) as a model system to: 1) validate tests of fish welfare for use in the field (indicators include metabolic scope, cortisol release rate, attention bias, and behavioral expression), and 2) test the hypothesis that fish stranded in fragmented areas experience different welfare outcomes compared to those in connected river regions.
Effect of an invasive competitor on the welfare of a threatened fish in a soft-release program
Grantee: Rafael Freire
Institutions: Charles Sturt University
Project summary
Invasive fish are common in most freshwater systems, yet their impact on the welfare of resident fish is often ignored. Invasive fish species compete and displace small-bodied native fish in several ways, including by preventing access to refuge sites, preferred foods, breeding grounds, and other important resources. This project will assess the welfare of native fish placed in sites with and without invasive competitors. Welfare will be measured using a novel population-level judgement bias test, differences in telomere attrition, and typical physical correlates of welfare in fish. In addition to revealing the impact of invasive fish on small-bodied fish welfare, this work will also promote greater consideration of the impact of animal translocation and release activities on wild animal welfare.
Integrating nonlethal field and lab assessments of wild fish welfare in the Colorado River in Grand Canyon
Grantee: Isaac Schuman
Institution: Oregon State University
Project summary
This project will monitor the welfare of three species of fish in the Grand Canyon using a battery of metrics including body condition, gut microbiome, parasite load, and cortisol in skin mucus. The researchers will attempt to validate these as welfare indicators by testing whether they correlate with one another within individual fish, and whether they follow consistent patterns across the three focal species. Finally, they will use long-term population monitoring data to investigate potential correlations between these individual-based welfare indicators and environmental and demographic characteristics of individual study sites, such as food availability and age structure.
Are populations that are well-adapted to their environment less stressed than those that are not?
Grantee: Ryan S. Mohammed
Institution: Auburn University
Project summary
In this pilot study, wild-caught guppies will be exposed to an apparent threat of predation as researchers transfer water from a tank containing a common predator (pike cichlid) into their tank. The amount of water transferred from the predators’ tank will be varied to simulate a range of predator densities. Cortisol levels will then be measured in the guppies’ tissue and tank water. These tissue and water cortisol measurements will be compared to develop a protocol for inferring the cortisol levels of fish based on non-invasive water measurements. The researchers hypothesize that the strength of the guppies’ physiological stress response (cortisol) will vary with apparent predation risk.
How do human activities impair the welfare of highly social fish?
Grantee: Joachim Frommen
Institution: Manchester Metropolitan University
Project summary
This project will investigate the welfare of a common fish species in Lake Tanganyika, the princess cichlid, as they are exposed to varying levels of human activity. Across eight populations representing a range of distances to human settlements and shipping routes, the researchers will monitor behaviors indicative of stress or aggression and measure body condition and the brain tissue expression of five genes involved in stress physiology (glucocorticoid response pathway; crf, cyp11b, gr1, gr2, mr). These welfare indicators will be compared with environmental characteristics including boat noise, water visibility (sedimentary and algal load), human fishing intensity, temperature stress, and structural complexity of the local environment.
Assessing the anthropogenic impacts, long-term health, and welfare of elasmobranch species within San Francisco Bay, California
Grantee: Meghan Holst
Institution: University of California, Davis
Project summary
The San Francisco Bay Estuary is used by individuals belonging to several elasmobranch populations during critical periods of their life history. But it is also dredged, used as a major harbor for ships, and fished, with sharks and their prey targeted. This project will measure stress physiology and blood contaminants to evaluate whether the health and welfare of elasmobranchs is threatened within San Francisco Bay. It will also use stable isotope analysis to evaluate the dietary needs and sensitivities of San Francisco Bay’s elasmobranch species to determine whether individuals are threatened with hunger due to fishing, and to map relationships within the ecosystem.
The impact of anthropogenic stressors on the affective state of juvenile Murray cod
Grantee: Rafael Freire
Institution: Charles Sturt University
Project summary
Many freshwater fish populations have severely declined as a result of human-caused changes in their environment. Population decline often results from an increased mortality rate experienced on the individual level, with likely implications for the welfare of individuals living through a period of population decline. This study will examine how differences in water quality and the presence of potential predators affect a behavioral indicator of welfare — judgment bias — in juvenile Murray cod (Maccullochella peelii). In the future, data about how juvenile fish respond to these factors could guide interventions for helping juvenile fish survive to adulthood in the wild.