Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Validating welfare indicators in an arachnid and their relationship to leg loss, a common defense strategy
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago, US
Grant amount: $29,946
Grant type: Discovery grants
Focal species: Several species of the arachnid order Opiliones in the genus Prionostemma (family Sclerosomatidae)
Conservation status: Not evaluated
Disciplines: Animal behavior, sentience
Research locations: Costa Rica, United States
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Why we funded this project
If validated, these behaviours will provide a novel set of welfare indicators in an arachnid, a group whose welfare has historically been neglected. The researchers’ previous work also shows that autotomy is very common in this taxon, so this project has the potential to help researchers understand the welfare of a very large number of individuals.
Remote welfare assessment in wildlife using stand-off Raman spectroscopy
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln
Project summary
This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln, UK
Grant amount: $55,519
Grant type: Discovery grants
Focal species: Tufted capuchin monkeys (Sapajus apella)
Conservation status: Least concern
Disciplines: Physiology, animal behavior
Research locations: United Kingdom, United States
Project summary
To enhance the welfare of wild animals, it is crucial to continuously monitor biomarkers and other metrics that can indicate changes in their welfare. However, this presents the challenge of obtaining repeated measurements from individuals, which often involves capture, restraint, and/or handling — procedures that can have significant negative welfare impacts on free-living wild animals. This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Why we funded this project
This project builds on a previous WAI-funded project, which validated the use of Raman spectroscopy as an efficient way of testing hormones in hair. It will test whether this method can be used to measure hair cortisol levels from a distance, potentially helping to make the use of this indicator more scalable for long-term monitoring in the field, and reducing the need for distressing or invasive methods for welfare assessment.
Assessing the Impact of Ectoparasites on Nestling Welfare: Validating Behavioral and Physiological Indicators in Darwin’s Finches
Grantee: Sabine Tebbich
Institution: University of Vienna
Project summary
Nestling finches are particularly vulnerable to blood-sucking ectoparasites because they lack the ability to preen, dust-bathe, or escape infested nests. Invasive parasites are especially detrimental to bird welfare because naïve hosts lack behavioral adaptations such as nest sanitation or preening, and hosts often suffer from exceptionally high parasite loads. This project aims to integrate behavioral (breathing rate and sleep duration) and physiological (haematocrit, baseline corticosterone, oxidative stress and telomere length) indicators to assess the welfare impact of the bloodsucking larvae of the avian vampire fly (Philornis downsi) on nestlings of Galápagos finches.
Grantee: Sabine Tebbich
Institution: University of Vienna, Austria
Grant amount: $42,000
Grant type: Discovery grants
Focal species: Green warbler-finches (Certhidae olivacea) and small ground finches (Geospiza fuliginosa)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, ornithology, infectious disease
Research locations: Austria, United States, Ecuador
Project summary
Nestling finches are particularly vulnerable to blood-sucking ectoparasites because they lack the ability to preen, dust-bathe, or escape infested nests. Invasive parasites are especially detrimental to bird welfare because naïve hosts lack behavioral adaptations such as nest sanitation or preening, and hosts often suffer from exceptionally high parasite loads. This project aims to integrate behavioral (breathing rate and sleep duration) and physiological (haematocrit, baseline corticosterone, oxidative stress and telomere length) indicators to assess the welfare impact of the bloodsucking larvae of the avian vampire fly (Philornis downsi) on nestlings of Galápagos finches.
Why we funded this project
We appreciate the diversity of welfare indicators this project will bring to bear on an important threat to the welfare of juveniles (an especially abundant and neglected life stage) in this species. By examining correlations between these putative welfare indicators, the project will contribute to understanding their validity, both individually and as a combined index, with potential transferability to other species and research questions.
A quest for a non-lethal method to assess spiders’ welfare in the urban environment
Grantee: Alessandra Costanzo
Institution: University of Milan
Project summary
This pilot project aims to develop welfare indicators for the orb-weaver spider Araneus angulatus, a species commonly found in urban settings. Juveniles will be exposed to urban stressors such as non-lethal mosquito-repellent pesticides, elevated temperatures simulating the Urban Heat Island effect, and changes in food availability. Once they reach adulthood, the effects of these stressors on individuals will be assessed using physiological (biomarkers of oxidative stress, detoxification, neurotoxicity, and energy metabolism) and morphological (body size, mass, and fluctuating asymmetry) indicators, and behavioral endpoints (prey capture rates and web structure). By integrating these measures, the project aims to triangulate spider welfare across multiple domains and improve our understanding of how urban stressors affect individual well-being.
Grantee: Alessandra Costanzo
Institution: University of Milan, Italy
Grant amount: $27,000
Grant type: Discovery grants
Focal species: Orb-weaver (Araneidae) and long-jawed orb-weaver spiders (Tetragnathidae)
Conservation status: n/a
Disciplines: Physiology, animal behavior
Research locations: Italy
Project summary
Urban areas are the fastest-growing habitat type worldwide, causing significant loss of other types of habitats and biodiversity declines, while also potentially reducing the welfare of some individual animals through sublethal stress. While urban wildlife research has mainly focused on charismatic species like birds and pollinators, less attention has been given to neglected taxa such as spiders. This pilot project aims to develop welfare indicators for the orb-weaver spider Araneus angulatus, a species commonly found in urban settings. Juveniles will be exposed to urban stressors such as non-lethal mosquito-repellent pesticides, elevated temperatures simulating the Urban Heat Island effect, and changes in food availability. Once they reach adulthood, the effects of these stressors on individuals will be assessed using physiological (biomarkers of oxidative stress, detoxification, neurotoxicity, and energy metabolism) and morphological (body size, mass, and fluctuating asymmetry) indicators, and behavioral endpoints (prey capture rates and web structure). By integrating these measures, the project aims to triangulate spider welfare across multiple domains and improve our understanding of how urban stressors affect individual well-being.
Why we funded this project
This project advances wild animal welfare science by introducing a multi-indicator framework for assessing welfare in an arachnid, a taxonomic group largely neglected in welfare research.
Examining mechanistic relationships between metabolic rates, wild bird welfare and impacts of selective logging on eastern Himalayan birds
Grantee: Akshay Bharadwaj
Institution: Indian Institute of Science
Project summary
Animals must balance energy spent with energy consumed, and can only persist in habitats that allow for this balance. All warm-blooded animals have a Basic Survival Cost (BSC) required for basal metabolism and thermoregulation. This project aims to understand how basic survival costs (BSC) affect wild bird welfare in the Eastern Himalayas. Using a combination of thermal imaging, respirometry, and ambient-temperature humidity data, we will estimate BSC in free-living birds across habitat gradients. Subsequently, the project will relate the BSC to multiple welfare indicators, allowing a triangulation of a bird's lived experience. By linking energy metabolism with welfare outcomes, this work will provide a novel, mechanistic perspective on wild animal welfare in an understudied tropical system.
Grantee: Akshay Bharadwaj
Institution: Indian Institute of Science, India
Grant amount: $37,500
Grant type: Discovery grants
Focal species: Understory insectivorous/frugivorous birds including the yellow-throated fulvetta, rusty-fronted barwing, and coral-billed scimitar babbler
Conservation status: n/a
Disciplines: Physiology, ornithology
Research locations: India
Project summary
Animals must balance energy spent with energy consumed, and can only persist in habitats that allow for this balance. All warm-blooded animals have a Basic Survival Cost (BSC) required for basal metabolism and thermoregulation. This project aims to understand how basic survival costs (BSC) affect wild bird welfare in the Eastern Himalayas. Using a combination of thermal imaging, respirometry, and ambient-temperature humidity data, we will estimate BSC in free-living birds across habitat gradients. Subsequently, the project will relate the BSC to multiple welfare indicators, allowing a triangulation of a bird's lived experience. By linking energy metabolism with welfare outcomes, this work will provide a novel, mechanistic perspective on wild animal welfare in an understudied tropical system.
Why we funded this project
This project builds on previous WAI-funded research and will provide insight into the relationship between welfare and energy expenditure, a commonly measured ecological parameter. Better understanding this relationship could prove useful in anticipating the welfare impacts of ecological changes, such as selective logging (the direct priority of this study).
Effect of an invasive competitor on the welfare of a threatened fish in a soft-release program
Grantee: Rafael Freire
Institutions: Charles Sturt University
Project summary
Invasive fish are common in most freshwater systems, yet their impact on the welfare of resident fish is often ignored. Invasive fish species compete and displace small-bodied native fish in several ways, including by preventing access to refuge sites, preferred foods, breeding grounds, and other important resources. This project will assess the welfare of native fish placed in sites with and without invasive competitors. Welfare will be measured using a novel population-level judgement bias test, differences in telomere attrition, and typical physical correlates of welfare in fish. In addition to revealing the impact of invasive fish on small-bodied fish welfare, this work will also promote greater consideration of the impact of animal translocation and release activities on wild animal welfare.
Grantee: Rafael Freire
Institution: Charles Sturt University, Australia
Grant amount: $20,200
Grant type: Discovery grants
Focal species: Southern pygmy perch (Nannoperca australis)
Conservation status: Endangered
Disciplines: Physiology, animal behavior, ichthyology, cognition
Research locations: Australia
Project summary
Invasive fish are common in most freshwater systems, yet their impact on the welfare of resident fish is often ignored. Invasive fish species compete and displace small-bodied native fish in several ways, including by preventing access to refuge sites, preferred foods, breeding grounds, and other important resources. This project will assess the welfare of native fish placed in sites with and without invasive competitors. Welfare will be measured using a novel population-level judgement bias test developed in 2023 with prior Wild Animal Initiative support. Additional measures of welfare will include differences in telomere attrition and typical physical correlates of welfare in fish. In addition to revealing the impact of invasive fish on small-bodied fish welfare, this work will also promote greater consideration of the impact of animal translocation and release activities on wild animal welfare.
Why we funded this project
This project builds on previous work funded by WAI and has immediate potential to inform freshwater systems restoration strategies with a welfare perspective. We are also pleased to see high-quality and scalable behavioral welfare indicators being developed for fish, a group whose welfare has historically been neglected and especially challenging to study.