Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Does DNA methylation reflect environmental and social adversity?
Grantee: Daniel T. Blumstein
Institution: University of California, Los Angeles
Project summary
This project will investigate the welfare effects of early cumulative adversity in free-living yellow-bellied marmots. It will look for associations between the adversity index and behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, the propensity to emit alarm calls while foraging); biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios); and two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Grantees: Daniel T. Blumstein, Emily Renkey
Institution: University of California, Los Angeles, US
Grant amount: $219,900
Grant type: Challenge grants
Focal species: Yellow-bellied marmot (Marmota flaviventer)
Conservation status: Least concern
Disciplines: Population ecology, genetics/genomics, ecological modeling, physiology
Research locations: United States
Project summary
In humans, early cumulative adversity has demonstrable consequences for health, welfare, and longevity. The welfare consequences of early adversity in wild animals is less well understood, possibly reflecting insufficient measures for assessment. Using a recently validated approach to quantifying cumulative adversity in free-living yellow-bellied marmots (Marmota flaviventer), this project will investigate the welfare effects of early cumulative adversity. It will look for associations between the adversity index and key behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, and the propensity to emit alarm calls while foraging), biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios), and ultimately, two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Why we funded this project
This project will add welfare to the research portfolio of a long-running study system of a free-living mammal. It will contribute to understanding the validity of biological aging as a welfare indicator by pairing it with other indicators and a comprehensive dataset of the animals’ adverse early-life experiences.
Harnessing machine learning for the non-invasive assessment of wild fish welfare
Grantee: Thomas Pike
Institution: University of Lincoln
Project summary
This project aims to develop an open-source aquatic camera system capable of automatically and non-invasively quantifying a suite of behavioural and cognitive welfare indicators in wild fish. Behavioural indicators include the degree of physical closeness between individuals within a group, the spatial arrangement of subgroups, movement behaviour, and agonistic interactions. The project will examine variation in these indicators along an urban–rural gradient to a range of putative stressors, before validating them using “challenge” tests involving ecologically relevant experimental interventions (e.g., acoustic disturbances such as boat noise). The overarching objective is to develop an open-source toolkit that integrates commercially available hardware with custom-designed software, allowing the methods to be readily used by others to assess wild fish welfare.
Grantee: Thomas Pike
Institution: University of Lincoln, UK
Grant amount: $81,927
Grant type: Challenge grants
Focal species: Fish
Conservation status: Least concern
Disciplines: Animal behavior, cognition, climate science, ichthyology
Research locations: United Kingdom
Project summary
Wild fish are facing unprecedented challenges from climate-change-induced alterations to natural environments and anthropogenic stressors. Yet assessing their welfare is extremely challenging. To address this, this project aims to develop an open-source aquatic camera system capable of automatically and non-invasively quantifying a suite of behavioural and cognitive welfare indicators. Behavioural indicators include the degree of physical closeness between individuals within a group, the spatial arrangement of subgroups, movement behaviour, and agonistic interactions. The project will examine variation in these indicators along an urban–rural gradient to a range of putative stressors, before validating them using “challenge” tests involving ecologically relevant experimental interventions (e.g., acoustic disturbances such as boat noise). The overarching objective is to develop an open-source toolkit that integrates commercially available hardware with custom-designed software, allowing the methods to be readily used by others to easily, cheaply, and repeatedly assess the welfare of wild fish.
Why we funded this project
This project will provide proof of concept for a novel approach to monitoring fish welfare at scale that is designed to be widely generalisable and translatable across species, habitats, and contexts. This project will improve current strategies for assessing the welfare of wild fish, most notably by avoiding the need for capture, handling, or restraint, and by being entirely non-invasive and non-disruptive.
Stranding: a blessing or a curse? Testing assumptions of fish welfare during habitat fragmentation
Grantee: Laura R. Stein
Institution: University of Oklahoma
Project summary
Fragmentation of freshwater habitats due to drought and heatwaves poses significant risks to aquatic organisms, particularly small fish, who are often overlooked in animal welfare studies. While fragmentation is generally considered detrimental to fish welfare — leading to stress, reduced social interactions, and increased mortality — there is also potential for positive welfare effects, such as reduced predation pressure or temperature-induced metabolic benefits. This project will use threespine stickleback (Gasterosteus aculeatus) as a model system to: 1) validate tests of fish welfare for use in the field (indicators include metabolic scope, cortisol release rate, attention bias, and behavioral expression), and 2) test the hypothesis that fish stranded in fragmented areas experience different welfare outcomes compared to those in connected river regions.
Grantees: Laura R. Stein, Justine Rionach McCarthy
Institution: University of Oklahoma, US
Grant amount: $136,365
Grant type: Challenge grants
Focal species: Threespine stickleback (Gasterosteus aculeatus)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, ichthyology
Research locations: United States
Project summary
Fragmentation of freshwater habitats due to drought and heatwaves poses significant risks to aquatic organisms, particularly small fish, who are often overlooked in animal welfare studies. While fragmentation is generally considered detrimental to fish welfare — leading to stress, reduced social interactions, and increased mortality — there is also potential for positive welfare effects, such as reduced predation pressure or temperature-induced metabolic benefits. This project will use threespine stickleback (Gasterosteus aculeatus) as a model system to: 1) validate tests of fish welfare for use in the field (indicators include metabolic scope, cortisol release rate, attention bias, and behavioral expression), and 2) test the hypothesis that fish stranded in fragmented areas experience different welfare outcomes compared to those in connected river regions.
Why we funded this project
This project will integrate multiple welfare indicators to evaluate behavioral and physiological responses, contributing to a deeper understanding of how environmental fragmentation impacts individual fish welfare, and developing non-invasive field methods for use on small fish species. This project also supports a Wild Animal Initiative fellow, PhD candidate Rionach McCarthy, who has a strong interest in welfare.
Validating welfare indicators in an arachnid and their relationship to leg loss, a common defense strategy
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago, US
Grant amount: $29,946
Grant type: Discovery grants
Focal species: Several species of the arachnid order Opiliones in the genus Prionostemma (family Sclerosomatidae)
Conservation status: Not evaluated
Disciplines: Animal behavior, sentience
Research locations: Costa Rica, United States
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Why we funded this project
If validated, these behaviours will provide a novel set of welfare indicators in an arachnid, a group whose welfare has historically been neglected. The researchers’ previous work also shows that autotomy is very common in this taxon, so this project has the potential to help researchers understand the welfare of a very large number of individuals.
Remote welfare assessment in wildlife using stand-off Raman spectroscopy
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln
Project summary
This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Grantees: Jose Gonzalez-Rodriguez and Teresa Romero
Institution: University of Lincoln, UK
Grant amount: $55,519
Grant type: Discovery grants
Focal species: Tufted capuchin monkeys (Sapajus apella)
Conservation status: Least concern
Disciplines: Physiology, animal behavior
Research locations: United Kingdom, United States
Project summary
To enhance the welfare of wild animals, it is crucial to continuously monitor biomarkers and other metrics that can indicate changes in their welfare. However, this presents the challenge of obtaining repeated measurements from individuals, which often involves capture, restraint, and/or handling — procedures that can have significant negative welfare impacts on free-living wild animals. This project aims to develop a stand-off Raman spectroscopy system as a non-invasive tool to assess hormonal levels in wild animals, which can be integrated into welfare assessments. The project also aims to validate under standardized and natural conditions how biomarkers of long-term stress relate to a range of stressors that may affect wild animal welfare. They will use a combination of socio-positive (e.g., play, grooming) and negative (e.g., aggression, screaming) behaviors, indices of social integration, behavioral indicators of anxiety (i.e., self-directed behaviors), and resting behavior, as well as physical indicators of welfare (body condition, instances of injury).
Why we funded this project
This project builds on a previous WAI-funded project, which validated the use of Raman spectroscopy as an efficient way of testing hormones in hair. It will test whether this method can be used to measure hair cortisol levels from a distance, potentially helping to make the use of this indicator more scalable for long-term monitoring in the field, and reducing the need for distressing or invasive methods for welfare assessment.
Assessing the Impact of Ectoparasites on Nestling Welfare: Validating Behavioral and Physiological Indicators in Darwin’s Finches
Grantee: Sabine Tebbich
Institution: University of Vienna
Project summary
Nestling finches are particularly vulnerable to blood-sucking ectoparasites because they lack the ability to preen, dust-bathe, or escape infested nests. Invasive parasites are especially detrimental to bird welfare because naïve hosts lack behavioral adaptations such as nest sanitation or preening, and hosts often suffer from exceptionally high parasite loads. This project aims to integrate behavioral (breathing rate and sleep duration) and physiological (haematocrit, baseline corticosterone, oxidative stress and telomere length) indicators to assess the welfare impact of the bloodsucking larvae of the avian vampire fly (Philornis downsi) on nestlings of Galápagos finches.
Grantee: Sabine Tebbich
Institution: University of Vienna, Austria
Grant amount: $42,000
Grant type: Discovery grants
Focal species: Green warbler-finches (Certhidae olivacea) and small ground finches (Geospiza fuliginosa)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, ornithology, infectious disease
Research locations: Austria, United States, Ecuador
Project summary
Nestling finches are particularly vulnerable to blood-sucking ectoparasites because they lack the ability to preen, dust-bathe, or escape infested nests. Invasive parasites are especially detrimental to bird welfare because naïve hosts lack behavioral adaptations such as nest sanitation or preening, and hosts often suffer from exceptionally high parasite loads. This project aims to integrate behavioral (breathing rate and sleep duration) and physiological (haematocrit, baseline corticosterone, oxidative stress and telomere length) indicators to assess the welfare impact of the bloodsucking larvae of the avian vampire fly (Philornis downsi) on nestlings of Galápagos finches.
Why we funded this project
We appreciate the diversity of welfare indicators this project will bring to bear on an important threat to the welfare of juveniles (an especially abundant and neglected life stage) in this species. By examining correlations between these putative welfare indicators, the project will contribute to understanding their validity, both individually and as a combined index, with potential transferability to other species and research questions.
A quest for a non-lethal method to assess spiders’ welfare in the urban environment
Grantee: Alessandra Costanzo
Institution: University of Milan
Project summary
This pilot project aims to develop welfare indicators for the orb-weaver spider Araneus angulatus, a species commonly found in urban settings. Juveniles will be exposed to urban stressors such as non-lethal mosquito-repellent pesticides, elevated temperatures simulating the Urban Heat Island effect, and changes in food availability. Once they reach adulthood, the effects of these stressors on individuals will be assessed using physiological (biomarkers of oxidative stress, detoxification, neurotoxicity, and energy metabolism) and morphological (body size, mass, and fluctuating asymmetry) indicators, and behavioral endpoints (prey capture rates and web structure). By integrating these measures, the project aims to triangulate spider welfare across multiple domains and improve our understanding of how urban stressors affect individual well-being.
Grantee: Alessandra Costanzo
Institution: University of Milan, Italy
Grant amount: $27,000
Grant type: Discovery grants
Focal species: Orb-weaver (Araneidae) and long-jawed orb-weaver spiders (Tetragnathidae)
Conservation status: n/a
Disciplines: Physiology, animal behavior
Research locations: Italy
Project summary
Urban areas are the fastest-growing habitat type worldwide, causing significant loss of other types of habitats and biodiversity declines, while also potentially reducing the welfare of some individual animals through sublethal stress. While urban wildlife research has mainly focused on charismatic species like birds and pollinators, less attention has been given to neglected taxa such as spiders. This pilot project aims to develop welfare indicators for the orb-weaver spider Araneus angulatus, a species commonly found in urban settings. Juveniles will be exposed to urban stressors such as non-lethal mosquito-repellent pesticides, elevated temperatures simulating the Urban Heat Island effect, and changes in food availability. Once they reach adulthood, the effects of these stressors on individuals will be assessed using physiological (biomarkers of oxidative stress, detoxification, neurotoxicity, and energy metabolism) and morphological (body size, mass, and fluctuating asymmetry) indicators, and behavioral endpoints (prey capture rates and web structure). By integrating these measures, the project aims to triangulate spider welfare across multiple domains and improve our understanding of how urban stressors affect individual well-being.
Why we funded this project
This project advances wild animal welfare science by introducing a multi-indicator framework for assessing welfare in an arachnid, a taxonomic group largely neglected in welfare research.
Examining mechanistic relationships between metabolic rates, wild bird welfare and impacts of selective logging on eastern Himalayan birds
Grantee: Akshay Bharadwaj
Institution: Indian Institute of Science
Project summary
Animals must balance energy spent with energy consumed, and can only persist in habitats that allow for this balance. All warm-blooded animals have a Basic Survival Cost (BSC) required for basal metabolism and thermoregulation. This project aims to understand how basic survival costs (BSC) affect wild bird welfare in the Eastern Himalayas. Using a combination of thermal imaging, respirometry, and ambient-temperature humidity data, we will estimate BSC in free-living birds across habitat gradients. Subsequently, the project will relate the BSC to multiple welfare indicators, allowing a triangulation of a bird's lived experience. By linking energy metabolism with welfare outcomes, this work will provide a novel, mechanistic perspective on wild animal welfare in an understudied tropical system.
Grantee: Akshay Bharadwaj
Institution: Indian Institute of Science, India
Grant amount: $37,500
Grant type: Discovery grants
Focal species: Understory insectivorous/frugivorous birds including the yellow-throated fulvetta, rusty-fronted barwing, and coral-billed scimitar babbler
Conservation status: n/a
Disciplines: Physiology, ornithology
Research locations: India
Project summary
Animals must balance energy spent with energy consumed, and can only persist in habitats that allow for this balance. All warm-blooded animals have a Basic Survival Cost (BSC) required for basal metabolism and thermoregulation. This project aims to understand how basic survival costs (BSC) affect wild bird welfare in the Eastern Himalayas. Using a combination of thermal imaging, respirometry, and ambient-temperature humidity data, we will estimate BSC in free-living birds across habitat gradients. Subsequently, the project will relate the BSC to multiple welfare indicators, allowing a triangulation of a bird's lived experience. By linking energy metabolism with welfare outcomes, this work will provide a novel, mechanistic perspective on wild animal welfare in an understudied tropical system.
Why we funded this project
This project builds on previous WAI-funded research and will provide insight into the relationship between welfare and energy expenditure, a commonly measured ecological parameter. Better understanding this relationship could prove useful in anticipating the welfare impacts of ecological changes, such as selective logging (the direct priority of this study).
Swan Lake: Assessing the welfare of urban mute swans
Grantee: Irena Uzelac
Institution: Animal Rescue Serbia
Project summary
This project investigates how human-provided food, environmental conditions, and injuries affect the welfare of mute swans in urban Belgrade. The project will assess welfare using indicators such as body condition, visible deformities, injury rates, and behavior patterns like foraging and aggression. By comparing sites with different levels of human feeding and variations in temperature, the project aims to understand how these factors shape swan health and behavior. Reproductive success and juvenile survival will also be monitored to evaluate long-term welfare outcomes. A mix of behavioral observation, environmental monitoring, and welfare scoring will be used, integrating both natural and anthropogenic stressors. The ultimate goal is to develop ecologically valid welfare indicators that reflect real-world challenges faced by urban wildlife.
Grantee: Irena Uzelac
Institution: Animal Rescue Serbia, Serbia
Grant amount: $10,000
Grant type: Seed grants
Focal species: Mute swan (Cygnus olor)
Conservation status: Least concern
Disciplines: Ornithology, animal behavior, physiology
Research locations: Serbia
Project summary
This project investigates how human-provided food, environmental conditions, and injuries affect the welfare of mute swans (Cygnus olor) in urban Belgrade. The project will assess welfare using indicators such as body condition, visible deformities (e.g., angel wing), injury rates, and behavior patterns like foraging and aggression. By comparing sites with different levels of human feeding and variations in temperature, the project aims to understand how these factors shape swan health and behavior. Reproductive success and juvenile survival will also be monitored to evaluate long-term welfare outcomes. A mix of behavioral observation, environmental monitoring, and welfare scoring will be used, integrating both natural and anthropogenic stressors. The ultimate goal is to develop ecologically valid welfare indicators that reflect real-world challenges faced by urban wildlife.
Why we funded this project
This project will help address the question of how food from humans and urban environmental stressors affect the health and welfare of wild birds by applying practical welfare indicators in a free-living, urban population of mute swans. While this question has been longstanding, most research has been limited to narrower health (disease) or demographic impacts. We hope that this project will provide information on when and how anthropogenic food might be a welfare threat or opportunity.
Validating body posture as a novel marker of well-being in animals
Grantee: Nicole Koyama
Institution: Liverpool John Moores University
Project summary
A few studies on nonhuman primates have found that a hunched posture is a response to social separation and physical inflammation, but research linking whole body posture and physiological measures of welfare is lacking. This project will combine established measures of affective valence (nasal temperature) and arousal (iris-pupil ratio) to validate body posture as a new measure of affective valence. Two months of video data will be collected and used for thermal imaging, behavioral, and postural analysis from groups of wild Barbary macaques, along with pilot data for comparison from non-human primate zoo animals. Ultimately, the project aims to share a new validated measure and conceptual framework that can be applied to a range of wild mammals.
Grantee: Nicola Koyama
Institution: Liverpool John Moores University, United Kingdom
Grant amount: $9,995
Grant type: Seed grants
Focal species: Barbary macaque (Macaca sylvanus)
Conservation status: Endangered
Disciplines: Primatology, animal behavior, physiology
Research locations: United Kingdom, Morocco
Project summary
A few studies on non-human primates have found that a hunched posture is a response to social separation and physical inflammation, but research linking whole body posture and physiological measures of welfare is lacking. This project will combine established measures of affective valence (nasal temperature) and arousal (iris-pupil ratio) to validate body posture as a new measure of affective valence. Two months of video data will be collected and used for thermal imaging, behavioral, and postural analysis from groups of wild Barbary macaques, along with pilot data for comparison from non-human primate zoo animals. Ultimately, the project aims to share a new validated measure and conceptual framework that can be applied to a range of wild mammals.
Why we funded this project
This project will validate an indicator for assessing welfare that could be relevant across primates, and provide proof of concept for its application in the field.