Meet our grantees

Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.

The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.

We showcase our grantees and their projects here and continuously update this page as new projects are added.

Does diet mediate effects of sublethal parasitic infections on host welfare?

Grantee: Amanda Koltz

Institution: University of Texas at Austin

Project summary

This project will test how infection by parasitic worms (helminths) influences host welfare in white-footed deer mice by evaluating the relationship between parasite burden and host body condition, microbiome, and stress physiology, as well as behaviors associated with anxiety (negative welfare) and exploration (positive welfare). The researchers will experimentally manipulate parasite burden by intervening to apply anti-parasitic medication (Ivermectin) as a treatment for some mice who were already infected with helminths.

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Developing an automated cognitive bias task for wild squirrels

Grantees: Vikki Neville, Lisa Leaver

Institutions: University of Bristol, University of Exeter

Project summary

The goal of this project is to develop a cognitive judgment bias test for use in wild gray squirrels that capitalizes on their innate behavior, avoiding the need for the extensive type of training that is usually part of cognitive judgment bias tests in captive settings. By making use of the widely available Raspberry Pi platform, the test should be relatively easy for other researchers to replicate and adapt for use in other species. To validate the test itself, the research team will also assess how measurement of cognitive judgment bias varies with factors assumed to affect the valence of animals participating in the test, such as distance from cover and levels of food provisioning in the environment, as well as how the test results correlate with other potential non-invasive behavioral and physiological indicators of welfare, such as the distance at which a squirrel flees from approaching humans, hair cortisol concentration, and real-time fluctuations in eye temperature in response to stimuli during the the test.

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Integrating behavioral competency and post-release support for reintroduced wildlife: a shift in paradigm for rehabilitation and beyond

Grantee: Karli Rice Chudeau

Institution: The Marine Mammal Center, University of California, Davis

Project summary

This project investigates post-release support and monitoring to improve outcomes for rehabilitated juvenile pinnipeds. Post-release support will include familiar cognitive enrichment to help released animals adjust gradually and buffer their affective state. Post-release monitoring will consider metrics such as behavioral diversity, energy expenditure, and body condition, and the animals’ specific behavioral profiles will be compared with those recorded from healthy, wild individuals. These metrics will be used to evaluate the effectiveness of post-release enrichment as an intervention for improving welfare outcomes. Cognitive bias tests for affective state carried out during rehabilitation and prior to release will also be considered as potential predictors of post-release welfare.

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Fellowship, Least concern, Mammals, United States, 2023, Not published Wild Animal Initiative Fellowship, Least concern, Mammals, United States, 2023, Not published Wild Animal Initiative

Street smarts and bold behaviors: How humans and urban environments influence the welfare of wild mesocarnivores

Grantee: Lauren Stanton

Institution: University of California, Berkeley

Project summary

This project will evaluate the welfare impacts of urban landscapes and human-wildlife conflict scenarios, focusing on environmental contamination and anthropogenic disturbance by assessing how they introduce poor diet and diseases that impact wild animals’ health and behavior, potentially increasing the likelihood of further conflict. It will use motion-activated infrared trail cameras, a novel method, to noninvasively assess and compare the behavior, cognition, and health of urban wildlife in relation to differences in environmental conditions, establishing an individual-based assessment of welfare. The project will also provide a welfare vulnerability assessment in relation to environmental characteristics, which will facilitate modeling and predicting welfare risk according to environmental variables, as well as identifying mitigation opportunities for reducing poor-welfare urban environments.

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Fellowship, Least concern, Sweden, Mammals, 2023, Not published Wild Animal Initiative Fellowship, Least concern, Sweden, Mammals, 2023, Not published Wild Animal Initiative

Quantifying the impact of sea ice coverage on the welfare of grey seal pups

Grantee: Daire Carroll

Institution: University of Gothenburg

Project summary

This project will use historic necropsy assessment and drone-based photogrammetry to establish and validate welfare proxies and remote behavioral and body conditions in grey seals as a remote welfare assessment tool. It will compare cortisol levels, behavior, and body condition of juvenile seals in land and ice breeding colonies at different densities to understand density-dependent welfare in relation to differences in environmental conditions. This information will be used to develop predictive models to identify welfare risk factors and opportunities to mitigate them. Opportunities to alleviate stress for juveniles during land breeding years will be identified and proposed. The project will demonstrate proof-of-concept for a combined population modeling and behavioral/health assessment approach that can be transferred to other species.

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Empirical assessment of welfare in wild American mink and Eurasian otters: the effects of intra- and inter-specific population density

Grantees: Lauren A Harrington, Maria Diez Leon

Institution: Oxford University

Project summary

This project seeks to determine whether the welfare of American mink is negatively impacted in the presence of high densities of Eurasian otters, whether both species’ welfare is compromised at relatively higher densities of conspecifics, and whether there is a seasonality to welfare impacts. The project will also assess whether behavioral time budget shifts in mink are associated with higher chronic stress levels as a proxy for negative impact on mink welfare. These questions will be addressed by measuring welfare through several different domains, including behavioral (exploratory behavior, vocalizations), physical (body condition, ectoparasite load), and physiological (telomere length, fecal glucocorticoid metabolites and hair cortisol) metrics.

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Determination of Fecal Tri-iodothyronine and Cortisol as Physiological Proxies of Animal Welfare

Grantees: Michael Cherry, Joe Hediger

Institutions: Caesar Kleberg Wildlife Research Institute, Texas A&M University, University of Massachusetts Dartmouth

Project summary

Wild animals are susceptible to the effects of thermal stress imposed by a warming climate, including increased energetic costs to maintain a healthy body temperature, immune system impairment, changes in food availability, and increases in disease transmission. White-tailed deer in southern Texas are on the front lines of this challenge. This project aims to assess the reliability of fecal tri-iodothyronine (T3) and fecal glucocorticoids (FGC) as non-invasive physiological metrics for monitoring their health. The use of T3 as an indicator of wildlife health and welfare is relatively novel, and the researchers will attempt to refine it through controlled experiments, correlating the T3 measurements with a more widely used indicator in FGC. Both physiological indicators will be validated against behavioral observations.

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Characterization of fecal oxytocin and immunoglobulin A in lions (Panthera leo): Exploring a multi-biomarker approach in animal welfare research

Grantee: Paula Serres Corral

Institution: Universitat Autònoma de Barcelona

Project summary

This project will validate the measurement of oxytocin (OT) and secretory immunoglobulin A (IgA), markers of neuroendocrine and immune function, as biomarkers of wild animals’ welfare states. Both indicators have been measured in the saliva and urine of a limited set of wild mammalian species, but work on their analysis in fecal samples has been even more limited. This project will validate the measurement of OT and IgA in the feces of lions as a model for social carnivores, and evaluate their applicability as additional physiological indicators in wild animal welfare studies. The researchers will evaluate baseline levels for the species based on a small number of captive individuals and assess how these biomarkers are correlated with GCs and behavior.

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Developing loop-mediated isothermal amplification (LAMP) assays for detecting pathogens in wild animal populations

Grantee: Cameron Semper

Institutions: University of Calgary, University of Lethbridge

Project summary

This project aims to develop Loop-mediated isothermal amplification (LAMP) assays for rapid, in situ detection of representative viral and bacterial pathogens, as well as parasitic worm infections in wild animals. This project will develop two methods for detecting viral and bacterial infections, respectively. The viral test will focus on viruses that have caused epidemics in wild frigatebird and sooty tern populations, while the bacterial test will focus on tick-borne illnesses and parasitic worms which infect deer mice. After developing the LAMP assays, the researchers will validate their potential contribution to monitoring wild animal welfare in a non-invasive manner by using them to test for pathogenic load in fecal samples from deer mice.

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Raman spectroscopy as a novel non-invasive technique to assess wildlife welfare

Grantee: Teresa Romero

Institutions: University of Lincoln, University of Portsmouth

Project summary

Analysis of hormones accumulated in the hair has emerged as a non-invasive tool for measuring chronic stress in wildlife, but hair analyses are currently limited by variation in cortisol concentrations. This project will test whether Raman spectroscopy, which is suitable for the field and does not require sample pre-treatment, is a better way to use hair samples to assess steroid hormones. The planned methodology will make use of samples of mammalian hair with a known cortisol content using a control technique — liquid chromatography mass spectrometry — for comparison. The samples will be subjected to analysis by Raman to validate the technique and establish a robust analytical methodology for the non-invasive analysis of welfare biomarkers in wildlife.

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