Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Acoustic indicators of welfare in neotropical frogs: Calls as early-warning signals of sublethal suffering related to infection and environmental stress
Grantee: Erin Wall
Institution: Wavelength Animal Communication Science and University of Texas at Austin, United States
Project summary
Emerging evidence indicates that frog call characteristics are modulated by environmental and physiological conditions, offering the potential for rapid insights into an individual’s welfare. This project will examine the relationship between environmental conditions, physiology, and behavior to connect indices of welfare to variation in acoustic signals in neotropical frogs in Panama. By linking these measures to natural levels of sublethal infection and variation in environmental conditions, the researchers will aim to identify acoustic signatures of welfare and develop a rapid, non-intrusive welfare detection tool for thousands of species of vocalizing frogs.
Grantee: Erin Wall
Institution: Wavelength Animal Communication Science and University of Texas at Austin, United States
Grant amount: $180,000
Grant type: Fellowship
Focal species: Túngara frog (Engystomops pustulosus), hourglass treefrog (Dendropsophus ebraccatus), leaf-litter toad (Rhinella alata), and others
Conservation status: Least concern
Disciplines: Herpetology, physiology, animal behavior
Research locations: Canada, Panama, United States
Project summary
Climate change, habitat loss, and infectious disease are creating compounding challenges for frogs, yet we have limited tools to monitor the behavioral and physiological consequences of those challenges, assess frog welfare, or detect sublethal suffering. Emerging evidence indicates that frog call characteristics are modulated by environmental and physiological conditions, offering the potential for rapid insights into an individual’s welfare. This project will examine the relationship between environmental conditions, physiology, and behavior to connect indices of welfare to variation in acoustic signals in neotropical frogs in Panama. By linking these measures to natural levels of sublethal infection and variation in environmental conditions, we aim to identify acoustic signatures of welfare and develop a rapid, non-intrusive welfare detection tool for thousands of species of vocalizing frogs.
Why we funded this project
Along with supporting an early-career researcher, this grant will deliver a behavioral assessment framework and acoustic classifier tool for anurans to deepen our understanding of welfare in this diverse taxon and address the need for evidence-based, scalable welfare assessment methods.
Environmental drivers of welfare in urban rats: A multimodal field study of micro-habitat conditions and affective behavior
Grantee: Emily Mackevicius
Institution: Basis Research Institute, United States
Project summary
This project will investigate how urban micro-habitat conditions shape the affective and social lives of brown rats (Rattus norvegicus). Better tools for understanding rat behavior could support both wild animal welfare science and integrative management practices. The project will first test whether indicators developed in laboratory rodents such as ultrasonic vocalizations, posture, movement, social behavior, and surface temperature can be used to infer affective state and social experience in urban field settings. The team will then model how environmental features predict these indicators, with the goal of identifying urban conditions associated with positive or negative experiences.
Grantee: Emily Mackevicius
Institution: Basis Research Institute, United States
Grant amount: $100,000
Grant type: Challenge grants
Focal species: Brown rat (Rattus norvegicus) and others
Conservation status: Least concern
Disciplines: Wildlife management, physiology, animal behavior
Research location: United States
Project summary
This project will investigate how urban micro-habitat conditions shape the affective and social lives of free-ranging brown rats (Rattus norvegicus). Rats are highly social, intelligent, and abundant animals, and better tools for understanding their behavior could support both wild animal welfare science and integrative management practices. The project will first test whether indicators developed in laboratory rodents such as ultrasonic vocalizations, posture, movement, social behavior, and surface temperature, can be used to infer affective state and social experience in urban field settings. The team will then model how environmental features predict these indicators, with the goal of identifying urban conditions associated with positive or negative experiences.
Why we funded this project
This project addresses a neglected but important question: how fine-scale features of human-built environments shape the subjective experiences of abundant urban animals. The resulting methods could support future welfare studies across sites, cities, and taxa, and provide tools for welfare-aware, evidence-based urban management.
Feeling salty: A welfare framework for estuarine fishes
Grantee: Samantha Levell
Institution: New College of Florida, United States
Project summary
Estuaries are highly dynamic environments in which salinity can change rapidly. This project will study how short-term, ecologically realistic salinity shifts influence welfare in estuarine fishes using non-invasive measures such as waterborne cortisol, metabolic rate, exploration, shoaling behavior, and decision making. The project will focus on two common estuarine species: the sheepshead minnow (Cyprinodon variegatus) and the sailfin molly (Poecilia latipinna). By comparing juveniles and adults, the project aims to develop practical tools for assessing fish welfare in the wild and to better understand how environmental variability shapes the experiences of aquatic animals.
Grantee: Samantha Levell
Institution: New College of Florida, United States
Grant amount: $26,200
Grant type: Discovery grants
Focal species: Sheepshead minnow (Cyprinodon variegatus), sailfin molly (Poecilia latipinna), and others
Conservation status: N/A: Multiple focal species
Disciplines: Ichthyology, physiology, animal behavior
Research location: United States
Project summary
Estuaries are highly dynamic environments in which salinity can change rapidly. This project will study how short-term, ecologically realistic salinity shifts influence welfare in estuarine fishes using non-invasive measures such as waterborne cortisol, metabolic rate, exploration, shoaling behavior, and decision making. The project will focus on two common estuarine species: the sheepshead minnow (Cyprinodon variegatus) and the sailfin molly (Poecilia latipinna). By comparing juveniles and adults, the project aims to develop practical tools for assessing fish welfare in the wild and to better understand how environmental variability shapes the experiences of aquatic animals.
Why we funded this project
Most previous work on estuarine fishes has focused on survival and physiological tolerance rather than the animals’ lived experiences or affective states. By integrating non-invasive physiological and behavioral indicators, this project will help validate a practical framework for assessing fish welfare under ecologically realistic conditions.
Validating acoustic monitoring as a non-invasive welfare indicator in urban coyotes: Linking vocalizations to health status, social dynamics, and environmental quality
Grantee: Caroline Rowley
Institution: Duke University, United States
Project summary
Urban coyotes face welfare challenges including disease, parasites, habitat degradation, reliance on anthropogenic food sources, and stress from human-wildlife conflict. This project is a pilot study aimed at validating passive acoustic monitoring as a welfare assessment tool. Data will be collected via GPS collars fitted with microphones, autonomous recording units, thermal camera traps and biological sampling. Using models and machine learning, the researchers will test whether vocal features correlate with established welfare indicators and whether individual coyotes can be identified from their howls alone. The aim is to develop a scalable, non-invasive framework for monitoring the welfare of free-ranging animals.
Grantee: Caroline Rowley
Institution: Duke University, United States
Grant amount: $49,978
Grant type: Discovery grants
Focal species: Eastern coyote (Canis latrans)
Conservation status: Least concern
Disciplines: Animal behavior, mammalogy
Research location: United States
Project summary
Coyote populations have expanded dramatically across North America, but population-level success does not necessarily equate to good welfare. Urban coyotes in particular face welfare challenges including disease, parasites, habitat degradation, reliance on anthropogenic food sources, and stress from human-wildlife conflict. This project is a pilot study aimed at validating passive acoustic monitoring as a welfare assessment tool. Data will be collected via GPS collars fitted with microphones, autonomous recording units, thermal camera traps and biological sampling. Using models and machine learning, the researchers will test whether vocal features correlate with established welfare indicators and whether individual coyotes can be identified from their howls alone. The goal is to develop a scalable, non-invasive framework for monitoring the welfare of free-ranging animals.
Why we funded this project
By systematically linking acoustic features of coyote vocalizations to established welfare indicators across multiple domains, this study positions passive acoustic monitoring as a scalable, whole-animal welfare assessment tool that is likely transferable to other similar species using vocalizations for communication.
Evaluating ice nests: Effects of a welfare intervention on long-lived insect societies and their guests
Grantee: Christina Kwapich
Institution: University of Central Florida, United States
Project summary
When social organisms are translocated, the destruction of multi-generational nests, loss of familiar territory, and disruption of demography could negatively influence the welfare of society members, future generations, and dependent symbionts. This project will translocate Florida harvester ant (Pogonomyrmex badius) colonies by burying replica nests made from ice. The team will measure risk-averse behaviors in ants across generations, as well as the stability of colony foraging routes, demography, and the re-colonization of symbiont populations. By monitoring ants in freshly transplanted and historically transplanted colonies, the project will provide data on the effects of social disruption on ant societies.
Grantee: Christina Kwapich
Institution: University of Central Florida, United States
Grant amount: $10,000
Grant type: Seed grants
Focal species: Florida harvester ant (Pogonomyrmex badius) and others
Conservation status: Not evaluated
Disciplines: Entomology, wildlife management
Research location: United States
Project summary
US laws increasingly require that species of interest be removed from land marked for development and translocated to suitable sites. For social organisms, the destruction of multi-generational nests, loss of familiar territory, and disruption of demography could negatively influence the welfare of surviving society members, future generations, and dependent symbionts. This project will translocate a population of Florida harvester ant (Pogonomyrmex badius) colonies by burying replica nests made from ice. The team will measure risk-averse behaviors in ants across generations, as well as the stability of colony foraging routes, demography, and the re-colonization of symbiont populations. By monitoring ants in freshly transplanted and historically transplanted colonies, the project will provide data on the effects of social disruption on long-lived animal societies made up of short-lived members.
Why we funded this project
The “ice nest” technique represents a welfare intervention that could mitigate some of the negative effects of social disruption associated with research or land development. By publishing on the feasibility of the ice nest technique, social insect well-being may be given serious consideration in future environmental mitigation and research protocols.
Does DNA methylation reflect environmental and social adversity?
Grantee: Daniel T. Blumstein
Institution: University of California, Los Angeles
Project summary
This project will investigate the welfare effects of early cumulative adversity in free-living yellow-bellied marmots. It will look for associations between the adversity index and behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, the propensity to emit alarm calls while foraging); biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios); and two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Grantees: Daniel T. Blumstein, Emily Renkey
Institution: University of California, Los Angeles, US
Grant amount: $219,900
Grant type: Challenge grants
Focal species: Yellow-bellied marmot (Marmota flaviventer)
Conservation status: Least concern
Disciplines: Population ecology, genetics/genomics, ecological modeling, physiology
Research locations: United States
Project summary
In humans, early cumulative adversity has demonstrable consequences for health, welfare, and longevity. The welfare consequences of early adversity in wild animals is less well understood, possibly reflecting insufficient measures for assessment. Using a recently validated approach to quantifying cumulative adversity in free-living yellow-bellied marmots (Marmota flaviventer), this project will investigate the welfare effects of early cumulative adversity. It will look for associations between the adversity index and key behaviors that indicate general wariness (flight initiation distance, time allocated to vigilance while foraging, and the propensity to emit alarm calls while foraging), biomarkers that indicate physiological stress (fecal glucocorticoid levels, neutrophil:lymphocyte ratios), and ultimately, two measures of aging (telomere length and DNA methylation). By quantifying these behavioral indices of wariness and the suite of biomarkers that culminate in telomeres and epigenetic state in pups throughout their first year and in older animals throughout their lives, the project will determine whether adverse environmental experiences have immediate and lasting effects on welfare.
Why we funded this project
This project will add welfare to the research portfolio of a long-running study system of a free-living mammal. It will contribute to understanding the validity of biological aging as a welfare indicator by pairing it with other indicators and a comprehensive dataset of the animals’ adverse early-life experiences.
Stranding: a blessing or a curse? Testing assumptions of fish welfare during habitat fragmentation
Grantee: Laura R. Stein
Institution: University of Oklahoma
Project summary
Fragmentation of freshwater habitats due to drought and heatwaves poses significant risks to aquatic organisms, particularly small fish, who are often overlooked in animal welfare studies. While fragmentation is generally considered detrimental to fish welfare — leading to stress, reduced social interactions, and increased mortality — there is also potential for positive welfare effects, such as reduced predation pressure or temperature-induced metabolic benefits. This project will use threespine stickleback (Gasterosteus aculeatus) as a model system to: 1) validate tests of fish welfare for use in the field (indicators include metabolic scope, cortisol release rate, attention bias, and behavioral expression), and 2) test the hypothesis that fish stranded in fragmented areas experience different welfare outcomes compared to those in connected river regions.
Grantees: Laura R. Stein, Justine Rionach McCarthy
Institution: University of Oklahoma, US
Grant amount: $136,365
Grant type: Challenge grants
Focal species: Threespine stickleback (Gasterosteus aculeatus)
Conservation status: Least concern
Disciplines: Physiology, animal behavior, ichthyology
Research locations: United States
Project summary
Fragmentation of freshwater habitats due to drought and heatwaves poses significant risks to aquatic organisms, particularly small fish, who are often overlooked in animal welfare studies. While fragmentation is generally considered detrimental to fish welfare — leading to stress, reduced social interactions, and increased mortality — there is also potential for positive welfare effects, such as reduced predation pressure or temperature-induced metabolic benefits. This project will use threespine stickleback (Gasterosteus aculeatus) as a model system to: 1) validate tests of fish welfare for use in the field (indicators include metabolic scope, cortisol release rate, attention bias, and behavioral expression), and 2) test the hypothesis that fish stranded in fragmented areas experience different welfare outcomes compared to those in connected river regions.
Why we funded this project
This project will integrate multiple welfare indicators to evaluate behavioral and physiological responses, contributing to a deeper understanding of how environmental fragmentation impacts individual fish welfare, and developing non-invasive field methods for use on small fish species. This project also supports a Wild Animal Initiative fellow, PhD candidate Rionach McCarthy, who has a strong interest in welfare.
Validating welfare indicators in an arachnid and their relationship to leg loss, a common defense strategy
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Grantee: Ignacio Escalante
Institution: University of Illinois, Chicago, US
Grant amount: $29,946
Grant type: Discovery grants
Focal species: Several species of the arachnid order Opiliones in the genus Prionostemma (family Sclerosomatidae)
Conservation status: Not evaluated
Disciplines: Animal behavior, sentience
Research locations: Costa Rica, United States
Project summary
This project will explore the welfare implications of leg loss, or “autotomy” — a common defensive strategy among animals. The project aims to validate welfare indicators within an understudied group of invertebrates, the Opiliones (Arachnida) in southwestern Costa Rica. Field and lab experiments will be used to test three potential behavioral welfare indicators: 1) movement patterns (approach vs. retreat), 2) speed, and 3) exploratory leg-tapping behaviors (count of leg taps) in response to positively and negatively valanced stimuli. These behaviors will be assessed when individuals are alone, in conspecific aggregations, and in barren versus complex housing. All experiments will include individuals with all legs and those with missing legs to assess how leg condition impacts welfare.
Why we funded this project
If validated, these behaviours will provide a novel set of welfare indicators in an arachnid, a group whose welfare has historically been neglected. The researchers’ previous work also shows that autotomy is very common in this taxon, so this project has the potential to help researchers understand the welfare of a very large number of individuals.
Comparing the welfare of stocked triploid vs native diploid rainbow trout in California
Grantee: Andrew Sharo
Institution: University of California, Los Angeles
Project summary
Rainbow trout are commonly stocked for recreational fishing throughout the world, but because of their negative impacts on the environment, many state agencies now stock triploid rainbow trout, which are sterile. However, triploid rainbow trout are known to be more sensitive to water temperature and dissolved oxygen, which may impact their welfare in wild environments. This project will use a combination of physiological and behavioral indicators to compare the welfare of diploid and triploid rainbow trout. We will assess the welfare of rainbow trout in ponds across the Los Angeles metropolitan region using qualitative behavioral assessment, neutrophil:lymphocyte ratio, body condition, injury/disease presence, and the expression of stressor-related genes, either non-invasively or through sampling fish caught by recreational anglers.
Grantee: Andrew Sharo
Institution: University of California, Los Angeles, US
Grant amount: $30,000
Grant type: Seed grant
Focal species: Rainbow/steelhead trout (Oncorhynchus mykiss)
Conservation status: Least concern
Disciplines: Ichthyology, genetics/genomics, physiology, animal behavior
Research locations: United States
Project summary
Rainbow trout are commonly stocked for recreational fishing throughout the world, but because of their negative impacts on the environment, many state agencies now stock triploid rainbow trout, which are sterile. However, triploid rainbow trout are known to be more sensitive to water temperature and dissolved oxygen, which may impact their welfare in wild environments. This project will use a combination of physiological and behavioral indicators to compare the welfare of diploid and triploid rainbow trout. We will assess the welfare of rainbow trout in ponds across the Los Angeles metropolitan region using qualitative behavioral assessment, neutrophil:lymphocyte ratio, body condition, injury/disease presence, and the expression of stressor-related genes, either non-invasively or through sampling fish caught by recreational anglers.
Does diet mediate effects of sublethal parasitic infections on host welfare?
Grantee: Amanda Koltz
Institution: University of Texas at Austin
Project summary
This project will test how infection by parasitic worms (helminths) influences host welfare in white-footed deer mice by evaluating the relationship between parasite burden and host body condition, microbiome, and stress physiology, as well as behaviors associated with anxiety (negative welfare) and exploration (positive welfare). The researchers will experimentally manipulate parasite burden by intervening to apply anti-parasitic medication (Ivermectin) as a treatment for some mice who were already infected with helminths.
Grantee: Amanda Koltz
Institutions: University of Texas at Austin, United States
Grant amount: $167,237
Grant type: Challenge grant
Focal species: White-footed mouse (Peromyscus leucopus) and their helminth parasites
Conservation status: Least concern
Disciplines: Infectious disease, animal welfare science, animal behavior, physiology, community ecology
Research location: United States
Project summary
Parasite-mediated changes in host traits can have far-reaching ecological effects. Even sublethal infections affect hosts by increasing energetic costs and altering behavior, immunity, and physiology. Yet while many studies have investigated parasite effects on specific host traits, our understanding of how parasites influence overall individual welfare is limited, especially for wild animals. For example, parasites can drive changes in host diet and habitat use that reduce parasite exposure but not necessarily improve other metrics of host welfare. A holistic approach that captures different types of individual-level responses to parasitism is needed to advance our overall understanding of sublethal infections on host welfare. We propose to investigate how parasite burden is associated with individual-level host welfare using white-footed deer mice (Peromyscus leucopus) and their helminth parasites as a model system. Specifically, by experimentally removing gastrointestinal helminth parasites from P. leucopus, we will test how variation in parasite burden influences individual host body condition, diet and nutrition, microbiome, stress physiology, anxiety-like behavior, and exploratory behavior in forested ecosystems. P. leucopus has become the dominant small mammal species over the last 40 years in the northern Great Lakes region. It experiences sublethal infection by a range of helminth parasites and is a reservoir for several zoonotic pathogens, making its host-parasite dynamics highly relevant to the health of humans and other wildlife. By examining how infection levels relate to the diet, body functions, and behavior of P. leucopus, this study will advance our understanding of how non-lethal parasitic infections affect the welfare of an extremely widespread wild animal species.
Why we funded this project
We are excited to fund a study on wild mice, a highly numerous and neglected group, and especially one with such a welfare-friendly experimental approach — curing parasitic infections rather than causing them. The project also uses a holistic suite of physiological and behavioral indicators that should allow the researchers to disentangle overall welfare from narrow, mechanistic impacts of infection on the health and nutrition domains.