Meet our grantees
Wild Animal Initiative funds academic research on high-priority questions in wild animal welfare.
The goal of our grants program is to fund research that deepens scientific knowledge of the welfare of wild animals in order to better understand how to improve the welfare of as many wild animals as possible, regardless of what causes the threats to their well-being.
We showcase our grantees and their projects here and continuously update this page as new projects are added.
Validation of non-invasive measures for life stage in wild octopuses and AI-Assisted Behavioral Analysis
Grantee: Michaella Andrade
Institution: Federal University of ABC
Project summary
This project aims to develop non-invasive methods to assess the life stage and welfare of wild octopuses. The project will develop an AI approach that learns to recognize complex behaviors, such as body patterns and ventilation rates, in video footage. In addition, the project will develop a methodology to automatically measure the life stage of octopuses in videos and non-invasive in situ measurements of the distance between the octopuses’ eyes and eyeballs. The main goal is to correlate these metrics with different behavioral contexts to determine whether they can be used as reliable indicators of the animal’s affective state. In the long term, the project aims to create an automated tool that accelerates and improves behavioral data analysis.
Grantee: Michaella Andrade
Institution: Federal University of ABC, Brazil
Grant amount: $10,000
Grant type: Seed grants
Focal species: Octopuses (Octopus insularis)
Conservation status: Not evaluated
Disciplines: Animal behavior
Research locations: Brazil
Project summary
This project aims to develop non-invasive methods to assess the life stage and welfare of wild octopuses. The project will develop an innovative AI approach that automatically learns to recognize complex behaviors, such as body patterns and ventilation rates, directly from pre-recorded videos in the field. In addition, the project will develop a methodology to automatically measure the life stage of octopuses in pre-recorded videos based on our database and non-invasive in situ measurements of the distance between the octopuses’ eyes and eyeballs. The main goal is to correlate these metrics with different behavioral contexts to determine whether they can be used as reliable indicators of the animal’s affective state. In the long term, the project aims to create an automated tool that accelerates and improves behavioral data analysis, enabling large-scale studies of the welfare of wild octopuses.
Why we funded this project
This project extends a previous WAI-funded project and uses innovative approaches to analyzing behavioral data for welfare assessment.
Effect of an invasive competitor on the welfare of a threatened fish in a soft-release program
Grantee: Rafael Freire
Institutions: Charles Sturt University
Project summary
Invasive fish are common in most freshwater systems, yet their impact on the welfare of resident fish is often ignored. Invasive fish species compete and displace small-bodied native fish in several ways, including by preventing access to refuge sites, preferred foods, breeding grounds, and other important resources. This project will assess the welfare of native fish placed in sites with and without invasive competitors. Welfare will be measured using a novel population-level judgement bias test, differences in telomere attrition, and typical physical correlates of welfare in fish. In addition to revealing the impact of invasive fish on small-bodied fish welfare, this work will also promote greater consideration of the impact of animal translocation and release activities on wild animal welfare.
Grantee: Rafael Freire
Institution: Charles Sturt University, Australia
Grant amount: $20,200
Grant type: Discovery grants
Focal species: Southern pygmy perch (Nannoperca australis)
Conservation status: Endangered
Disciplines: Physiology, animal behavior, ichthyology, cognition
Research locations: Australia
Project summary
Invasive fish are common in most freshwater systems, yet their impact on the welfare of resident fish is often ignored. Invasive fish species compete and displace small-bodied native fish in several ways, including by preventing access to refuge sites, preferred foods, breeding grounds, and other important resources. This project will assess the welfare of native fish placed in sites with and without invasive competitors. Welfare will be measured using a novel population-level judgement bias test developed in 2023 with prior Wild Animal Initiative support. Additional measures of welfare will include differences in telomere attrition and typical physical correlates of welfare in fish. In addition to revealing the impact of invasive fish on small-bodied fish welfare, this work will also promote greater consideration of the impact of animal translocation and release activities on wild animal welfare.
Why we funded this project
This project builds on previous work funded by WAI and has immediate potential to inform freshwater systems restoration strategies with a welfare perspective. We are also pleased to see high-quality and scalable behavioral welfare indicators being developed for fish, a group whose welfare has historically been neglected and especially challenging to study.
Between freedom and security, what makes a feral pigeon happier?
Grantee: Brandon Mak
Institution: Technical University of Munich
Project summary
Feral pigeons in Germany are subject to the Augsburg model of non-lethal population control, which involves attracting them to breed in dovecots where food and nest sites are provided, and their eggs exchanged with dummies. Others are captured and housed in aviaries under similar conditions. This project investigates the welfare implications of Germany’s non-lethal population control strategies. This is done by comparing the stress profiles of feral pigeons living “wild” in the streets, free-living pigeons breeding and feeding in dovecots, and formerly free-living pigeons translocated into aviaries. Fieldwork will be conducted to weigh pigeons (body mass measurement), collect egg and feather samples from their nests (corticosterone concentrations), and observe their behavior (locomotion and aggression).
Grantee: Brandon Mak
Institution: Technical University of Munich, Germany
Grant amount: $10,900
Grant type: Seed grants
Focal species: Feral pigeon/rock dove (Columba livia domestica)
Conservation status: Least concern
Disciplines: Ornithology, animal behavior, physiology, population ecology, wildlife management
Research locations: Germany
Project summary
Feral pigeons in Germany are subject to the Augsburg model of non-lethal population control, which involves attracting them to breed in dovecots where food and nest sites are provided, and their eggs exchanged with dummies. Others are captured and housed in aviaries under similar conditions. While aimed at reducing pigeon populations, providing food, and reducing exposure to predators, it’s possible that these measures may incidentally alleviate chronic stress in free-living individuals. This project investigates the welfare implications of Germany’s non-lethal population control strategies. This is done by comparing the stress profiles of feral pigeons living “wild” in the streets, free-living pigeons breeding and feeding in dovecots, and formerly free-living pigeons translocated into aviaries. Fieldwork will be conducted to weigh pigeons (body mass measurement), collect egg and feather samples from their nests (corticosterone concentrations), and observe their behavior (locomotion and aggression).
Why we funded this project
This project will contribute to our understanding of the potential welfare effects of (non-chemical) fertility control in pigeons, a leading candidate for near-term interventions to improve welfare in urban contexts. It will also provide some information on the validity of egg corticosterone as a non-invasive indicator of stress by measuring it alongside other more established methods (feather corticosterone, morphological and behavioural indicators) for a more robust welfare assessment.
Comparing the welfare of stocked triploid vs native diploid rainbow trout in California
Grantee: Andrew Sharo
Institution: University of California, Los Angeles
Project summary
Rainbow trout are commonly stocked for recreational fishing throughout the world, but because of their negative impacts on the environment, many state agencies now stock triploid rainbow trout, which are sterile. However, triploid rainbow trout are known to be more sensitive to water temperature and dissolved oxygen, which may impact their welfare in wild environments. This project will use a combination of physiological and behavioral indicators to compare the welfare of diploid and triploid rainbow trout. We will assess the welfare of rainbow trout in ponds across the Los Angeles metropolitan region using qualitative behavioral assessment, neutrophil:lymphocyte ratio, body condition, injury/disease presence, and the expression of stressor-related genes, either non-invasively or through sampling fish caught by recreational anglers.
Grantee: Andrew Sharo
Institution: University of California, Los Angeles, US
Grant amount: $30,000
Grant type: Seed grant
Focal species: Rainbow/steelhead trout (Oncorhynchus mykiss)
Conservation status: Least concern
Disciplines: Ichthyology, genetics/genomics, physiology, animal behavior
Research locations: United States
Project summary
Rainbow trout are commonly stocked for recreational fishing throughout the world, but because of their negative impacts on the environment, many state agencies now stock triploid rainbow trout, which are sterile. However, triploid rainbow trout are known to be more sensitive to water temperature and dissolved oxygen, which may impact their welfare in wild environments. This project will use a combination of physiological and behavioral indicators to compare the welfare of diploid and triploid rainbow trout. We will assess the welfare of rainbow trout in ponds across the Los Angeles metropolitan region using qualitative behavioral assessment, neutrophil:lymphocyte ratio, body condition, injury/disease presence, and the expression of stressor-related genes, either non-invasively or through sampling fish caught by recreational anglers.
The impact of winter food provisioning on the welfare of wild urban hedgehogs
Grantee: Julia Nowack
Institution: Liverpool John Moores University
Project summary
This project will investigate the welfare impacts of supplemental feeding during winter on European hedgehogs. Hedgehogs and other species have adapted to the lack of natural food during the winter by hibernating. Previous research, however, has suggested that hibernation may be interrupted when anthropogenic food sources are available. This project will use biologgers to collect data on winter activity and hibernation, as well as the animals’ use of specially designed feeding stations equipped with miniature thermal imaging devices, which will be used to non-invasively measure eye temperature as an indicator of physiological stress. Welfare assessment will also be supplemented by data on ectoparasite load, body mass, fecal glucocorticoid concentrations, latency to approach a feeding station, and survival rate for adults and juveniles.
Grantee: Julia Nowack
Institution: Liverpool John Moores University, UK
Grant amount: $29,977
Grant type: Seed grant
Focal species: Western European hedgehog (Erinaceus europaeus)
Conservation status: Least concern
Disciplines: Physiology, population ecology, animal behavior
Research locations: United Kingdom
Project summary
This project will investigate the welfare impacts of supplemental feeding during winter on European hedgehogs. Hedgehogs and other species have adapted to the lack of natural food during the winter by hibernating. Previous research, however, has suggested that hibernation may be interrupted when anthropogenic food sources are available. This project will use biologgers to collect data on winter activity and hibernation, as well as the animals’ use of specially designed feeding stations equipped with miniature thermal imaging devices, which will be used to non-invasively measure eye temperature as an indicator of physiological stress. Welfare assessment will also be supplemented by data on ectoparasite load, body mass, fecal glucocorticoid concentrations, latency to approach a feeding station, and survival rate for adults and juveniles.
Why we funded this project
Food provisioning is a potentially important near-term intervention that is already practiced in some contexts, but usually without data on or a primary interest in its wild animal welfare impacts. We appreciate that this project will use a non-invasive approach with multiple physiological and behavioral indicators to evaluate welfare impacts of supplemental feeding on wild European hedgehogs, including juveniles. The results could have implications for the management of other hibernators with access to supplementary feeding, such as dormice, bears, or chipmunks.
Field tests of bee welfare
Grantee: Vivek Nityanada
Institution: Newcastle University
Project summary
The goal of this project is to develop a cognitive judgment bias test for wild bees and pilot its use in the field. The project will investigate how bee cognitive biases and reward responses vary with factors expected to cause high or low welfare, such as light and vehicular pollution. The researchers will also develop a “lick-o-meter” for assessing welfare based on individual variation in the bees’ consumption of a sugar-water reward, with the expectation that bees in low-welfare environments will display less enthusiasm for consuming the sugar-water.
Grantee: Vivek Nityananda
Institutions: Newcastle University, United Kingdom
Grant amount: $194,317
Grant type: Challenge grant
Focal species: Buff tailed bumblebees (Bombus terrestris)
Conservation status: Least concern
Disciplines: Animal welfare science, entomology, animal behavior
Research location: United Kingdom
Project summary
Studying affective states in animals has been a key component towards assessing their welfare. Most studies that have used this approach have focused on vertebrates. Yet, the rapid decline in insect numbers calls out for novel methods to monitor their welfare and measuring their affective states would be an important approach. A few recent papers have indicated the presence of affective states in insects, especially bees. These papers have used judgement bias tests in laboratory settings. Recent findings using an active choice test in our lab have also shown robust evidence of affective states in bees due to changed expectations of rewards. However, there is no research looking at these states in the wild. To address this gap, this project seeks to develop and validate new tests for bee affective states in the wild and use these to assess the welfare of bees in the field.
This project will assess whether environments predicted to induce poor welfare and negative valence for bees– such as ones with poor nutrition or light pollution- induce changes in predicted markers of poor welfare. We will use performances in judgement bias tests and reduced responses to rewards in the wild as behavioral markers of welfare. We will also measure the role of neuromodulators (dopamine, serotonin) by measuring the differences in their expression, and of genes involved in their synthesis pathways, in the brains of wild bees in different environments. Measuring changes in these three different markers across different low-welfare environments will help validate them as measure of bee welfare and develop novel markers for wild bee welfare. They will thus providing vital tools for further biological and environmental research in a variety of pollinators.
Why we funded this project
We are generally excited to support this project because it focuses on a wild insect. Insects are extremely abundant and their welfare is typically neglected. Validating a cognitive judgment bias test that can be implemented in the field for a wild insect would be a powerful step forward.
Are we making urban wildlife sick?
Grantee: Carl Soulsbury
Institution: University of Lincoln
Project summary
The goal of this project is to test whether differences in welfare among individual red foxes and individual European hedgehogs are explained by specific environmental stressors arising from urbanization (e.g., increased human population density, noise pollution, or reduced habitat heterogeneity). Notably, on a population level, foxes appear to have fared well alongside humans, while hedgehogs have struggled to cope with urbanization. The study will confirm whether this contrast is also seen at the level of individual welfare, and offers a unique opportunity to identify common environmental stressors that may be impacting the health and welfare of urban wildlife more generally. To assess welfare, the project will perform cognitive judgment bias and novel object tests and combine them with non-invasive indicators of physiological stress (fecal glucocorticoids and eye/ear temperature) and health (pathogen load).
Grantee: Carl Soulsbury
Institutions: University of Lincoln, United Kingdom
Grant amount: $148,741.43
Grant type: Challenge grant
Focal species: Red fox (Vulpes vulpes) and European hedgehog (Erinaceus europaeus)
Conservation status: Least concern
Disciplines: Animal welfare science, animal behavior, physiology
Research location: United Kingdom
Project summary
Urbanisation causes profound changes to biodiversity. For many species living in urban areas, there is evidence that urban living leads to greater disease prevalence. It is unclear, however, (a) what factors drive this higher disease prevalence and (b) whether this leads to poorer wild animal welfare. We propose a study to compare two urban-dwelling species, red foxes and Eurasian hedgehogs, where we will characterize pathogen prevalence, load, and diversity across an urban-rural gradient. This study will be carried out at two scales: firstly, we will link pathogen loads, faecal glucocortoicoids and habitat at the landscape level, allowing us to broadly understand if urbanisation is associated with increased pathogen loads. We will then carry out a second, more detailed data collection where we will combine pathogen data with measures of stress (infra-red thermography, faecal glucocorticoids) and behavioural measures of affective valence (novel object and judgement bias tests). Here, we will link together pathogen loads and different measures of welfare at the individual level. Ultimately, our aim is to test, within each species, whether population differences in health and welfare are explained by environmental stressors arising from urbanisation (e.g., increased human population density and reduced habitat heterogeneity).
Why we funded this project
We appreciate that this project considers specific characteristics of urban environments, as opposed to simply comparing urban environments with rural ones. This way, it has more potential to inform near-term interventions. Plus, the project design humanely allows for animals’ voluntary participation. In addition to using an effective suite of welfare indicators to investigate the impact of different parasite loads on welfare, the combination of cognitive bias testing with non-invasive physiological stress metrics will provide information on the validity of those metrics, which are being used concurrently in other projects.
Does diet mediate effects of sublethal parasitic infections on host welfare?
Grantee: Amanda Koltz
Institution: University of Texas at Austin
Project summary
This project will test how infection by parasitic worms (helminths) influences host welfare in white-footed deer mice by evaluating the relationship between parasite burden and host body condition, microbiome, and stress physiology, as well as behaviors associated with anxiety (negative welfare) and exploration (positive welfare). The researchers will experimentally manipulate parasite burden by intervening to apply anti-parasitic medication (Ivermectin) as a treatment for some mice who were already infected with helminths.
Grantee: Amanda Koltz
Institutions: University of Texas at Austin, United States
Grant amount: $167,237
Grant type: Challenge grant
Focal species: White-footed mouse (Peromyscus leucopus) and their helminth parasites
Conservation status: Least concern
Disciplines: Infectious disease, animal welfare science, animal behavior, physiology, community ecology
Research location: United States
Project summary
Parasite-mediated changes in host traits can have far-reaching ecological effects. Even sublethal infections affect hosts by increasing energetic costs and altering behavior, immunity, and physiology. Yet while many studies have investigated parasite effects on specific host traits, our understanding of how parasites influence overall individual welfare is limited, especially for wild animals. For example, parasites can drive changes in host diet and habitat use that reduce parasite exposure but not necessarily improve other metrics of host welfare. A holistic approach that captures different types of individual-level responses to parasitism is needed to advance our overall understanding of sublethal infections on host welfare. We propose to investigate how parasite burden is associated with individual-level host welfare using white-footed deer mice (Peromyscus leucopus) and their helminth parasites as a model system. Specifically, by experimentally removing gastrointestinal helminth parasites from P. leucopus, we will test how variation in parasite burden influences individual host body condition, diet and nutrition, microbiome, stress physiology, anxiety-like behavior, and exploratory behavior in forested ecosystems. P. leucopus has become the dominant small mammal species over the last 40 years in the northern Great Lakes region. It experiences sublethal infection by a range of helminth parasites and is a reservoir for several zoonotic pathogens, making its host-parasite dynamics highly relevant to the health of humans and other wildlife. By examining how infection levels relate to the diet, body functions, and behavior of P. leucopus, this study will advance our understanding of how non-lethal parasitic infections affect the welfare of an extremely widespread wild animal species.
Why we funded this project
We are excited to fund a study on wild mice, a highly numerous and neglected group, and especially one with such a welfare-friendly experimental approach — curing parasitic infections rather than causing them. The project also uses a holistic suite of physiological and behavioral indicators that should allow the researchers to disentangle overall welfare from narrow, mechanistic impacts of infection on the health and nutrition domains.
Developing an automated cognitive bias task for wild squirrels
Grantees: Vikki Neville, Lisa Leaver
Institutions: University of Bristol, University of Exeter
Project summary
The goal of this project is to develop a cognitive judgment bias test for use in wild gray squirrels that capitalizes on their innate behavior, avoiding the need for the extensive type of training that is usually part of cognitive judgment bias tests in captive settings. By making use of the widely available Raspberry Pi platform, the test should be relatively easy for other researchers to replicate and adapt for use in other species. To validate the test itself, the research team will also assess how measurement of cognitive judgment bias varies with factors assumed to affect the valence of animals participating in the test, such as distance from cover and levels of food provisioning in the environment, as well as how the test results correlate with other potential non-invasive behavioral and physiological indicators of welfare, such as the distance at which a squirrel flees from approaching humans, hair cortisol concentration, and real-time fluctuations in eye temperature in response to stimuli during the the test.
Grantees: Vikki Neville and Lisa Leaver
Institutions: University of Bristol, University of Exeter, United Kingdom
Grant amount: $157,049
Grant type: Challenge grant
Focal species: Grey squirrel (Sciurus carolinensis)
Conservation status: Least concern
Disciplines: Animal welfare science, physiology, animal behavior
Research location: United Kingdom
Project summary
The development of the cognitive judgment bias (CJB) task for non-human animals revolutionised the field of animal welfare. The CJB task: (a) is thought to measure both relatively better and relatively poorer welfare across the full spectrum of possible welfare states, (b) is non-invasive, and (c) has been validated using a meta-analytic approach. Moreover, the theoretical basis of the predictions for the CJB task should be applicable across taxa and, accordingly, it has been used successfully in many species. However, to date, its use has largely been in captive species and there are a dearth of examples in wild animals. A key barrier to implementation is that it is difficult to train animals to associate stimuli with particular outcomes: the time needed to do so makes these studies unsuccessful or infeasible in non-captive populations of animals. We propose that this could be overcome by capitalising on the natural behaviour of a species to reduce training time and by using equipment that allows automation of stimulus presentation and data collection.
The main aim of our proposed project is to develop a CJB task for use in wild animals, in particular wild squirrels. Our key objectives are to develop a task for collecting CJB data from grey squirrels which: (1) capitalises on their innate behaviour, obviating the need for extensive training, and (2) makes use of Raspberry Pi equipment so that the task can be easily implemented by other researchers and straightforwardly adapted for use across species to measure welfare and validate novel potential measures of welfare.
To assess that our task works as anticipated, secondary objectives will be to: (1) assess how CJB varies with task manipulations designed to alter affective valence (the distance of the equipment from cover, and levels of food provisioning in the environment), and (2) assess how CJB correlates with other potential non-invasive indicators of welfare (e.g. flight distance in response to humans, QBA scores, retrapability, social status, hair cortisol concentration, and fluctuations in eye temperature following positive and negative stimuli on the judgement bias test measured using infrared thermal cameras).
Why we funded this project
As noted above, cognitive bias tests are generally considered to be exceptionally robust welfare indicators, while gray squirrels are an abundant species and a prime target for near-term interventions. Therefore, we are excited to support the development of a cognitive judgment bias test for use on free-living gray squirrels, which will also help to assess the validity of simpler indicators, such as eye temperature and flight initiation distance.
Validating the use of cognitive bias to assess affective valence in wild bird populations.
Grantee: Oliver Burman
Institution: University of Lincoln
Project summary
The goal of this project is to develop and validate a cognitive bias test for assessing the welfare of woodland birds that does not require any training stage, and instead takes advantage of innate attraction and aversion to stimuli that resemble preferred or noxious prey (with a range of likeness accuracy, based on the principle of imperfect Batesian mimicry). Continuing the theme of exploiting natural variation, the project will use spatial variation in numerous components of habitat preferability that can be assumed to directionally affect welfare — such as food abundance and shelter — as natural experiments to validate their cognitive bias tests.
Grantees: Oliver Burman
Institutions: University of Lincoln, United Kingdom
Grant amount: $193,684.17
Grant type: Challenge grant
Focal species: Free-living wild birds, including (but not limited to): blue tits (Cyanistes caeruleus), great tits (Parus major), wrens (Troglodytes troglodytes), coal tits (Poecile ater), European robins (Erithacus rubecula), chaffinches (Fringilla coelebs), greenfinches (Chloris chloris), starlings (Sturnus vulgaris), blackbirds (Turdus merula) and Eurasian jays (Garrulus glandarius).
Conservation status: Least concern
Disciplines: Animal welfare science, ornithology, animal behavior
Research location: United Kingdom
Project summary
Cognitive bias is a robust and validated measure of affective valence effective in a wide variety of animal species. However, this valuable approach has been primarily focused on the welfare assessment of captive animals and has not yet been demonstrated in wild free-living animal populations. This goal is crucial for addressing urgent contemporary issues such as the impact of anthropogenic and climatic change on wild animal welfare and to tackle key conservation challenges. In the first attempt to apply a cognitive judgement bias task in a free-living wild animal population, we found that wild birds struggled to complete the necessary training (discrimination) stage of the cognitive bias task. In the proposed project, our aim is therefore to validate two novel cognitive approaches to assessing affective valence in wild animals that do not require training, in conjunction with established behavioural and physiological indicators of welfare. Firstly, we will investigate a modified cognitive judgement bias task based on the innate avoidance of naturally occurring aversive prey, using the principle of imperfect Batesian mimicry. Secondly, we will modify and validate a cognitive attention bias task that relies on a spontaneous response to a potential threat. Both tasks will be assessed in populations of wild birds using natural variation in affective valence. Our anticipated outcomes are the development of an effective, validated measure of affective valence applicable to wild birds without the need for capture/restraint that can be translated across species; an invaluable tool in the assessment of wild animal welfare in response to a wide variety of environmental challenges.
Why we funded this project
This project’s results are intended to be generalizable to numerous woodland bird species (e.g., tits, wrens, starlings). We also appreciate its creatively humane approach to validation, using innate preferences and natural variation in affective valence, rather than training and experimental manipulation. Plus, the project considers both presumably positive (e.g., food, shelter) and presumably negative (e.g., predator abundance) welfare factors, which further strengthens the validation.